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KMID : 0370220230670040261
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Yakhak Hoeji
2023 Volume.67 No. 4 p.261 ~ p.267
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Identification of Ferroptosis Induction by Treating Atorvastatin in B16F10.OVA Cells
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Kim Min-Seok
Im In-Ho
Jeong Yun-Hwa
Kim Seung-Hyun
Cho Young-Chang
Choi Jeong-Uk
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Abstract
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In this study, we identified whether atorvastatin could induce ferroptosis or not in B16F10.OVA cells, whichis being used to treat hypercholesterolemia. To demonstrate this, we treated the mouse melanoma cell line B16F10.OVAwith atorvastatin at varying concentrations, spanning from low to high, followed by a 24-hour culture period. In the grouptreated with atorvastatin in our experiment, we observed a decrease in cell viability that was dependent on theconcentration of the treatment, when compared to the control group. The IC50 value of atorvastatin in B16F10.OVA cellswas determined to be 49.89 μM. Compared to the control group, treatment with atorvastatin resulted in a concentrationdependentincrease in the generation of reactive oxygen species and the accumulation of iron ions. Moreover, atorvastatintreatment exhibited a concentration-dependent increase in the population of apoptotic cells and the expression ofcalreticulin, significantly surpassing that of the control group. The analysis of ferroptosis-related protein expression levelsrevealed a notable decrease in the expression of GPX4 in the group treated with atorvastatin, as compared to the controlgroup. These findings strongly suggest that atorvastatin holds promise as a potent anti-cancer agent capable of inducingferroptosis in melanoma cells.
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KEYWORD
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Atorvastatin, Ferroptosis, Melanoma
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